Anti-PDE4D (phospho S190) antibody (ab59212)


  • Product nameAnti-PDE4D (phospho S190) antibody
    See all PDE4D primary antibodies
  • Description
    Rabbit polyclonal to PDE4D (phospho S190)
  • Specificityab59212 detects endogenous levels of PDE4D only when phosphorylated at serine 190.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190 (R-E-SP-F-L).

  • Positive control
    • IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab59212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 91 kDa).


  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D (phospho S190) antibody images

  • All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution

    Lane 1 : K562 cell extract treated with H2O2 (100uM, 30mins)
    Lane 2 : K562 cell extract treated with H2O2 (100uM, 30mins) with immunising peptide

    Predicted band size : 91 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

References for Anti-PDE4D (phospho S190) antibody (ab59212)

ab59212 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 25 µg
Specification heart
Gel Running Conditions Reduced Denaturing (4-12% bis tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 21 2012

Thank you for contacting Abcam.

We talked earlier on the phone about testing ab59212 in mouse via western blot. The mouse and human PDE4D protein have 98.5% homology; thus, this antibody is eligible for testing through our AbTrial program. Det...

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