The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 93 kDa.
Use at an assay dependent dilution.
RelevanceCyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
Cellular localizationCell Membrane and Cytoplasmic
Anti-PDE4D3 antibody (ab14614) at 1/500 dilution + Recombinant human PDE4D3 protein (ab80332) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 8 minutes
References for Anti-PDE4D3 antibody (ab14614)
This product has been referenced in:
Rababa'h A et al. Protein kinase A and phosphodiesterase-4D3 binding to coding polymorphisms of cardiac muscle anchoring protein (mAKAP). J Mol Biol425:3277-88 (2013).
Read more (PubMed: 23806656) »
Raymond DR et al. Distinct phosphodiesterase-4D variants integrate into protein kinase A-based signaling complexes in cardiac and vascular myocytes. Am J Physiol Heart Circ Physiol296:H263-71 (2009).
Read more (PubMed: 19060129) »