Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE4D3
  • Specificity
    ab14614 does not cross react with PDE4D1, D2, D4 and D5. Does not cross react with PDE4A, PDE4B or PDE4C.
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from unique region near N-terminal of the PDE4D3 variant.

  • Positive control
    • Tested with rat cortical membranes.

Properties

Applications

Our Abpromise guarantee covers the use of ab14614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 93 kDa.
IP 1/150.
ICC Use at an assay dependent dilution.

Target

  • Relevance
    Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
  • Cellular localization
    Cell Membrane and Cytoplasmic
  • Database links
  • Alternative names
    • DPDE3 antibody
    • hPDE4D3 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • PDE4D antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D antibody
    • Phosphodiesterase 4D cAMP-specific antibody
    • STRK1 antibody
    see all

Images

  • Anti-PDE4D3 antibody (ab14614) at 1/500 dilution + Recombinant human PDE4D3 protein (ab80332) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

This product has been referenced in:
  • Rababa'h A  et al. Protein kinase A and phosphodiesterase-4D3 binding to coding polymorphisms of cardiac muscle anchoring protein (mAKAP). J Mol Biol 425:3277-88 (2013). Read more (PubMed: 23806656) »
  • Raymond DR  et al. Distinct phosphodiesterase-4D variants integrate into protein kinase A-based signaling complexes in cardiac and vascular myocytes. Am J Physiol Heart Circ Physiol 296:H263-71 (2009). WB ; Human . Read more (PubMed: 19060129) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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