Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE4DIP
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 26-171 of Human PDE4DIP, isoform 2 (Q5VU43-2).

  • Positive control
    • Human duodenum tissue; RT4 and U251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function
    May function as an anchor sequestering components of the cAMP-dependent pathway to Golgi and/or centrosomes.
  • Tissue specificity
    Highly expressed in heart and skeletal muscle and to a lower extent in brain and placenta.
  • Involvement in disease
    Note=A chromosomal aberration involving PDE4DIP may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • Sequence similarities
    Contains 1 NBPF domain.
  • Developmental stage
    Expressed in fetal heart.
  • Cellular localization
    Golgi apparatus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiomyopathy associated protein 2 antibody
    • Cardiomyopathy-associated protein 2 antibody
    • CMYA2 antibody
    • MMGL antibody
    • MYOME_HUMAN antibody
    • Myomegalin antibody
    • Pde4dip antibody
    • Phosphodiesterase 4D interacting protein antibody
    • Phosphodiesterase 4D-interacting protein antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of A-431 cells labelling PDE4DIP with ab121375 shows positivity in the Golgi apparatus.

  • ab121375 at 1/15 dilution staining PDE4DIP in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-PDE4DIP antibody (ab121375) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab121375 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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