• Product nameAnti-PDE5A antibody
    See all PDE5A primary antibodies
  • Description
    Rabbit polyclonal to PDE5A
  • SpecificityThis antibody detects 99 and 89 kDa PDE5A proteins in various tissues.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PDE5A. Synthetic peptide common to all PDE5A variants, taken within amino acid region 120-170 of human PDE5A protein.
    (Peptide available as ab150691)

  • Positive control
    • Rat lung homogenate.
  • General notesStorage of very dilute antibody solutions is not recommended.



Our Abpromise guarantee covers the use of ab14672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500. Detects a band of approximately 89, 99 kDa (predicted molecular weight: 105 kDa).Can be blocked with Human PDE5A peptide (ab150691).
IP 1/250.


  • FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • PathwayPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • Post-translational
    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Anti-PDE5A antibody images

  • ab14672 (4µg/ml) staining PDE5A in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab14672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14672, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PDE5A antibody (ab14672)

This product has been referenced in:
  • van Heerebeek L  et al. Low myocardial protein kinase g activity in heart failure with preserved ejection fraction. Circulation 126:830-9 (2012). IHC-P ; Human . Read more (PubMed: 22806632) »
  • Sogawa C  et al. Gastrointestinal tract disorder in natriuretic peptide receptor B gene mutant mice. Am J Pathol 177:822-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20616347) »

See all 3 Publications for this product

Product Wall

Te pido disculpas por la espera.
El péptido usado como inmunógeno para generar ab14672 se ha añadido finalmente al catálogo.
El número de catálogo del péptido es ab150691.
Espero que con ayuda del péptido consigáis demostrar la especific...

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Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been succesful.

In order to help with our invesigation of this case before decidi...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your enquiry. With necrotic tissue, it is very likely that the proteins are already being broken down and degraded by the enzymes that are already present. I am sorry it would be very difficult to say and guarantee how much Endothelin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (right ventricle)
Specification right ventricle
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 12 2008

Thank you for your enquiry. The antigenic peptide was taken from amino acids 858-875, the peptide was modified and conjugated to carrier protein. The antibody was made in rabbit and was affinity purified using immobilized antigen based chromatograp...

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Thank you for your enquiry. At the moment, ab14672 is the only PDE5 antibody that we have. Ab14672 has been tested for application in Western blotting and IP and tested for cross-reactivity with Human, Mouse and Rat. If you decide to go ahead and pu...

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