Overview

  • Product name
  • Description
    Goat polyclonal to PDE5A
  • Specificity
    This antibody is expected to recognise all 4 reported isoforms (as represented by NP_001074; NP_236914; NP_246273). [NP_237223 has been retracted].
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-LINGESG QAKRN

    , corresponding to C terminal amino acids 864-875 of Human PDE5A.

  • Positive control
    • Human lung lysate. Lysate prepared from transfected HEK293 cells transiently expressing PDE5A.

Properties

Applications

Our Abpromise guarantee covers the use of ab28761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with PDE5A peptide (864-875) (ab45684).

Target

  • Function
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • Tissue specificity
    Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • Pathway
    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Domain
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • Post-translational
    modifications
    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Anti-PDE5A antibody images

  • Anti-PDE5A antibody (ab28761) at 0.3 µg/ml + Human lung lysate 30µg total protein per lane

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
  • Anti-PDE5A antibody (ab28761) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing PDE5A (isoform 2)

    Predicted band size : 100 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

References for Anti-PDE5A antibody (ab28761)

ab28761 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Grey seal Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Lung
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Verified customer

Submitted Mar 01 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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