Overview

  • Product nameAnti-PDE5A antibody
    See all PDE5A primary antibodies
  • Description
    Rabbit polyclonal to PDE5A
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human PDE5A.

    (Peptide available as ab66454.)

  • Positive control
    • This antibody gave a positive signal int he following Tissue Lysates: Mouse Skeletal Muscle, Human Ovary

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • PathwayPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • Post-translational
    modifications
    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase antibody
    • CGB PDE antibody
    • CGB-PDE antibody
    • CGBPDE antibody
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase antibody
    • cGMP binding cGMP specific phosphodiesterase antibody
    • cGMP binding/cGMP specific phosphodiesterase antibody
    • cGMP specific 3'5' cyclic phosphodiesterase antibody
    • cGMP specific phosphodiesterase PDE5A2 antibody
    • cGMP specific phosphodiesterase type 5A antibody
    • cGMP-binding cGMP-specific phosphodiesterase antibody
    • cGMP-specific 3'' antibody
    • CN5A antibody
    • CN5N antibody
    • PDE 5 antibody
    • PDE 5A antibody
    • PDE5 antibody
    • Pde5a antibody
    • PDE5A_HUMAN antibody
    • PDE5A1 antibody
    • Phosphodiesterase 5A antibody
    • Phosphodiesterase 5A cGMP specific antibody
    • Phosphodiesterase isozyme 5 antibody
    see all

Anti-PDE5A antibody images

  • All lanes : Anti-PDE5A antibody (ab64179) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : 42 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab64179 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64179, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 1µg/ml.
  • IHC image of ab64179 staining PDE5A in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Anti-PDE5A antibody (ab64179) at 1 µg/ml + Recombinant mouse PDE5A protein (ab80330) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-PDE5A antibody (ab64179)

ab64179 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (4-20% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Aug 14 2009

Application Western blot
Sample Rat Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (7.5 % mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (7.5 % mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Jul 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 15 µg
Specification platelet
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Platelet)
Specification Platelet
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"