Anti-PDE6 alpha antibody (ab85308)

Overview

  • Product nameAnti-PDE6 alpha antibody
    See all PDE6 alpha primary antibodies
  • Description
    Rabbit polyclonal to PDE6 alpha
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human PDE6 alpha.

    (Peptide available as ab95409.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; WERI; Y79.

Properties

Applications

Our Abpromise guarantee covers the use of ab85308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).

Target

  • FunctionThis protein participates in processes of transmission and amplification of the visual signal.
  • Involvement in diseaseDefects in PDE6A are the cause of retinitis pigmentosa type 43 (RP43) [MIM:613810]. RP43 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase subunit alpha antibody
    • CGPR A antibody
    • GMP PDE alpha antibody
    • GMP-PDE alpha antibody
    • PDE 6 alpha antibody
    • PDE 6A antibody
    • PDE V B1 antibody
    • PDE V-B1 antibody
    • PDE6A antibody
    • PDE6A_HUMAN antibody
    • PDEA antibody
    • Phosphodiesterase 6 alpha antibody
    • Phosphodiesterase 6A alpha subunit antibody
    • Phosphodiesterase 6A cGMP specific rod alpha antibody
    • Retinal Rod Photoreceptor cGMP Phosphodiesterase alpha antibody
    • Rod cGMP specific 3' 5' cyclic phosphodiesterase alpha subunit antibody
    • Rod cGMP-specific 3'' antibody
    see all

Anti-PDE6 alpha antibody images

  • All lanes : Anti-PDE6 alpha antibody (ab85308) at 1 µg/ml

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-PDE6 alpha antibody (ab85308)

ab85308 has not yet been referenced specifically in any publications.

Product Wall

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