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Our Abpromise guarantee covers the use of ab5663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa.Can be blocked with PDE6 beta peptide (ab5890).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17960120|
|IHC-Fr||Use at an assay dependent concentration.|
Western blot of PDE6 beta using ab5663.
Lane 1 demostrates no detection on extracts of RD mice.
Lane 2 shows a single band at ~90 kDa on Balb/C extracts.
Immunofluorescences on frozen secions of mouse retina outer segments after staining with ab5663.