Overview

  • Product nameAnti-PDE6 delta antibody
    See all PDE6 delta primary antibodies
  • Description
    Rabbit polyclonal to PDE6 delta
  • SpecificityThis antibody does not detect other PDE6 isoforms.
  • Tested applicationsSuitable for: ICC, ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse PDE6 delta aa 1-16.
    Sequence:

    MSAKDERAR(D/E)ILRGFK


    (Peptide available as ab41788)

  • Positive control
    • WB: bovine retinal extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10 - 1/200.
ICC/IF 1/10 - 1/200.
IHC-Fr Use a concentration of 1 µg/ml.

Immunohistochemical staining of PDE6 delta in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments.

WB Use a concentration of 2 µg/ml. Detects a band of approximately 17 kDa.Can be blocked with PDE6 delta peptide (ab41788).

Target

  • FunctionActs as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation.
  • Tissue specificityRetina.
  • Sequence similaritiesBelongs to the PDE6D/unc-119 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase subunit delta antibody
    • GMP PDE delta antibody
    • GMP-PDE delta antibody
    • p17 protein antibody
    • PDE 6 antibody
    • PDE 6D antibody
    • PDE D antibody
    • PDE6 antibody
    • PDE6D antibody
    • PDE6D_HUMAN antibody
    • PDED antibody
    • Phosphodiesterase 6D cGMP specific antibody
    • Phosphodiesterase 6D cGMP specific rod delta antibody
    • Protein p17 antibody
    • Retinal rod photoreceptor cGMP phosphodiesterase delta antibody
    • Retinal rod rhodopsin sensitive cGMP 3' 5' cyclic phosphodiesterase subunit delta antibody
    • Retinal rod rhodopsin-sensitive cGMP 3'' antibody
    see all

Anti-PDE6 delta antibody images

  • Immunocytochemistry/Immunofluorescence analysis of PDE6 delta (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescence analysis of PDE6 delta (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • This image is courtesy of an anonymous Abreview

    Ab5665 (1/750) staining PDE6 delta in MDCK cells
    Secondary antibody: An IRDye® Donkey anti-rabbit monoclonal (1/10000)
    Reduced denaturing conditions
    Blocking step: 10% Licor blocking buffer for 1 hour at 25°C

    See Abreview

References for Anti-PDE6 delta antibody (ab5665)

ab5665 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Liver cells,MDCK)
Loading amount 50 µg
Specification Liver cells,MDCK
Treatment siRNA against PDE6D in different concentrations
Gel Running Conditions Reduced Denaturing (4-20% Precise protein gels)
Blocking step Licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Nov 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"