Recombinant
RabMAb

Anti-PDE7B antibody [EPR11491] (ab170914)

Overview

  • Product name
    Anti-PDE7B antibody [EPR11491]
  • Description
    Rabbit Monoclonal [EPR11491] to PDE7B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PDE7B aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NP56

  • Positive control
    • MCF-7; HeLa; HepG2 and BxPC-3 cell lysates; Human fetal brain tissue lysate; HeLa cells; Human brain tissue; Human heart tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/250 - 1/500.

Antigen retrieval is recommended.

WB 1/1000 - 1/5000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      PDE7B is a cAMP specific phosphodiesterase. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis. PDE7B may be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.
    • Cellular localization
      cytosol
    • Database links
    • Alternative names
      • bA472E5.1 antibody
      • cAMP specific 3 5 cyclic phosphodiesterase 7B antibody
      • cAMP specific 3'5' cyclic phosphodiesterase 7B antibody
      • High affinity cAMP specific 3 5 cyclic phosphodiesterase antibody
      • High affinity cAMP specific 3'5' cyclic phosphodiesterase antibody
      • MGC88256 antibody
      • OTTHUMP00000017267 antibody
      • PDE 7B antibody
      • PDE7B protein antibody
      • Phosphodiesterase 7B antibody
      • Phosphodiesterase7B antibody
      • RNPDE7B antibody
      • Rolipram insensitive phosphodiesterase type 7 antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labeling PDE7B using ab170914 at 1/500 dilution.  DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling PDE7B with ab170914 at 1/100 dilution

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PDE7B with ab170914 at 1/100 dilution

    • Flow cytometric analysis of permeabilized Hela cells labeling PDE7B using ab170914 at 1/500 dilution  (red) or a rabbit IgG negative (green).

    • All lanes : Anti-PDE7B antibody [EPR11491] (ab170914) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : BxPC-3 cell lysate
      Lane 5 : Human fetal brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa

    References

    ab170914 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170914.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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