Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE8A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 779 and 829 of Human PDE8A (NP_002596.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
  • Tissue specificity
    Expressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 8A antibody
    • cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
    • FLJ16150 antibody
    • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody
    • High affinity cAMP-specific and IBMX-insensitive 3'' antibody
    • HsT19550 antibody
    • PDE 8A antibody
    • PDE8A antibody
    • PDE8A_HUMAN antibody
    • Phosphodiesterase 8A antibody
    • Phosphodiesterase8A antibody
    • Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
    see all

Images

  • All lanes : Anti-PDE8A antibody (ab109597) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg


    Predicted band size : 93 kDa
  • Detection of PDE8A by Western Blot of Immunprecipitate.
    Lane 1: ab109597 at 1µg/ml staining PDE8A in HeLa whole cell lysate immunoprecipitated using ab109597 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab109597 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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