Overview

  • Product nameAnti-PDE8A antibody
    See all PDE8A primary antibodies
  • Description
    Mouse monoclonal to PDE8A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: RLPQGQKTAA LPRTRGAGLL ESELRDGSGK KVAVADVQFG PMRFHQDQLQ VLLVFTKEDN QCNGFCRACE KAGFKCTVTK EAQAVLACFL, corresponding to amino acids 32-121 of human PDE8A (NP_002596) with a 26 kDa tag.

  • Positive control
    • PDE8A transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 93 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development.
  • Tissue specificityExpressed in most tissues except thymus and peripheral blood leukocytes. Highest levels in testis, ovary, small intestine and colon.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 8A antibody
    • cAMP specific cyclic nucleotide phosphodiesterase 8A antibody
    • FLJ16150 antibody
    • High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A antibody
    • High affinity cAMP-specific and IBMX-insensitive 3'' antibody
    • HsT19550 antibody
    • PDE 8A antibody
    • PDE8A antibody
    • PDE8A_HUMAN antibody
    • Phosphodiesterase 8A antibody
    • Phosphodiesterase8A antibody
    • Weakly similar to 3'5' cyclic nucleotide phosphodiesterase antibody
    see all

Anti-PDE8A antibody images

  • Anti-PDE8A antibody (ab77181) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 93 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PDE8A antibody (ab77181) at 5 µg/ml

    Lane 1 : PDE8A transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa

References for Anti-PDE8A antibody (ab77181)

ab77181 has not yet been referenced specifically in any publications.

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