The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 98-100 kDa.
Use at an assay dependent concentration. PubMed: 18431404
Use at an assay dependent concentration. This antibody is ideal for activity measurement and screening of various PDE8B enzyme variants in sample.
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland.
Tissue specificityAbundantly expressed in the thyroid. Also very weakly expressed in brain, spinal cord and placenta. In the thyroid isoform 1 predominates, and isoforms 2 and 6 are also highly expressed. In the placenta isoforms 1 and 2 are expressed equally. In the brain isoform 2 predominates.
Involvement in diseaseDefects in PDE8B are the cause of striatal degeneration autosomal dominant (ADSD) [MIM:609161]. It is a movement disorder affecting the striatal part of the basal ganglia and characterized by bradykinesia, dysarthria and muscle rigidity. These symptoms resemble idiopathic Parkinson disease, but tremor is not present.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily. Contains 1 PAS (PER-ARNT-SIM) domain.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Cell proliferation-inducing gene 22 protein antibody
High affinity cAMP specific and IBMX insensitive 3' 5' cyclic phosphodiesterase 8B antibody
High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8B antibody
High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B antibody
HSPDE 8B antibody
PDE 8B antibody
Phosphodiesterase 8B antibody
Anti-PDE8B antibody images
Western blot - PDE8B antibody (ab14621)
Western Blot analysis of PDE8B with ab14621 diluted 1:500. Lane 1 - rat testis; lane 2 - rat heart.
References for Anti-PDE8B antibody (ab14621)
This product has been referenced in:
Wilmot Roussel H et al. Identification of gene expression profiles associated with cortisol secretion in adrenocortical adenomas. J Clin Endocrinol Metab98:E1109-21 (2013).
Read more (PubMed: 23539725) »
Horvath A et al. A cAMP-specific phosphodiesterase (PDE8B) that is mutated in adrenal hyperplasia is expressed widely in human and mouse tissues: a novel PDE8B isoform in human adrenal cortex. Eur J Hum Genet16:1245-53 (2008).
Read more (PubMed: 18431404) »