Anti-PDGF AA antibody (ab9701)


  • Product name
  • Description
    Rabbit polyclonal to PDGF AA
  • Tested applications
    Suitable for: WB, ELISA, Neutralising, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hPDGF-AA (human Platelet Derived Growth Factor)



Our Abpromise guarantee covers the use of ab9701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPDGF-AA by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-AA.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (10.0 ng/ml), a concentration of 0.17 - 0.19 µg/ml of this antibody is required.
IHC-P Use a concentration of 1 µg/ml.


  • Function
    Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Domain
    The long form contains a basic insert which acts as a cell retention signal.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • PDGF A antibody
    • PDGF A chain antibody
    • PDGF subunit A antibody
    • PDGF-1 antibody
    • PDGF1 antibody
    • PDGFA antibody
    • PDGFA_HUMAN antibody
    • Platelet derived growth factor alpha chain antibody
    • Platelet derived growth factor alpha isoform 2 preproprotein antibody
    • Platelet derived growth factor alpha polypeptide antibody
    • Platelet-derived growth factor A chain antibody
    • Platelet-derived growth factor alpha polypeptide antibody
    • Platelet-derived growth factor subunit A antibody
    see all

Anti-PDGF AA antibody images

  • ab9701 at 1.0 µg/ml staining normal human skin by IHC-P. The antibody was incubated overnight at 4˚C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval.

References for Anti-PDGF AA antibody (ab9701)

ab9701 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Flow Cytometry
Human Cell (Natural Killer Cells)
Gating Strategy
Natural Killer Cells
Cell harvesting/tissue preparation method: Isolation from blood.
Sample buffer: RPMI
No fixation

Abcam user community

Verified customer

Submitted May 09 2017

Thank you for your email. Since the antibody has not been tested for cross-reactivity with mouse, we cannot guarantee that it will work. We do guarantee that an9701 will work with human samples in the following applications: Western blot, ELISA, Neutra...

Read More

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

Read More

All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. The immunogen used was recombinant hPDGF-AA so I am unable to check the homolgy. You may be ab...

Read More

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product. I would expect that this antibody would work in this application as it was raise...

Read More


Sign up