Overview

  • Product name
  • Description
    Goat polyclonal to PDGF B
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PDGF B (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KHRKFKHTHDKT

  • Positive control
    • IHC-P: Human pancreas and placenta tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23815774
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry Image of Human Pancreas tissue: Formalin Fixed and Paraffin Embedded (FFPE) and stained with anti PDGF B antibody (ab111310) at 3.75 µg/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

  • Immunohistochemistry Image of Human Placenta tissue: Formalin-Fixed and Paraffin-Embedded (FFPE) and stained with anti-PDGF B antibody (ab111310) at 3.75 µg/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

References

This product has been referenced in:
  • You W  et al. Foxc2 regulates osteogenesis and angiogenesis of bone marrow mesenchymal stem cells. BMC Musculoskelet Disord 14:199 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 23815774) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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