Overview

  • Product nameAnti-PDGF B antibody
    See all PDGF B primary antibodies
  • Description
    Rabbit polyclonal to PDGF B
  • Tested applicationsSuitable for: WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPDGF-BB by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03 - 0.04 µg/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration. To be used with ab84265 as detection antibody and ab9706 as protein standard.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Becaplermin antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • SIS antibody
    • SSV antibody
    see all

Anti-PDGF B antibody images

  • ab9704 staining PDGF BB in normal human skin tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent proteinase K mediated antigen retrieval. The primary antibody was used at 1 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen.

  • Anti-PDGF B antibody (ab9704) at 0.2 µg/ml + A431 (Human) Whole Cell Lysate (ab52255) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 18,29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible dimer).

References for Anti-PDGF B antibody (ab9704)

This product has been referenced in:
  • Yang J  et al. Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway. Blood 115:51-60 (2010). Neutralising ; Human . Read more (PubMed: 19880494) »
  • Kim GI  et al. Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode. Biosens Bioelectron 25:1717-22 (2010). Read more (PubMed: 20047825) »

See all 2 Publications for this product

Product Wall


The lab let me know that this antibody (ab84265, detection antibody) can be used with ab9704 as capture antibody and ab9706 as protein standard.

Thank you for contacting Abcam.
Earlier we discussed the specificity of ab9704 for detecting PDGF BB as well as the expected expression of PDGF BB across cell lines and tissues.
According to the human protein atlas, PDGF B (alternative name for...

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Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our othe...

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I have pasted the supplier's protocol below, but please do not hesitate to contact us should you have any other questions. Neutralization Assay for Anti-h-PDGF-BB using 3T3 cells Antibody: Anti-Human PDGF Procedure: 1) Resusp...

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Unfortunately, we do not have it in stock. However, from placing your order it will take approximately 2 working days for us to obtain this antibody, followed by another 24 hrs to ship the product to you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"