Overview

  • Product name
    Anti-PDGF B antibody [EPR6834]
    See all PDGF B primary antibodies
  • Description
    Rabbit monoclonal [EPR6834] to PDGF B
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep
  • Immunogen

    Synthetic peptide within Human PDGF B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P01127

  • Positive control
    • WB: Fetal brain, A375 and HeLa lysates. IHC-P: Human brain, heart and placenta tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab178409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    Images

    • All lanes : Anti-PDGF B antibody [EPR6834] (ab178409) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : A375 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 27 kDa
    • Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling PDGF B with ab178409 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling PDGF B with ab178409 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labeling PDGF B with ab178409 at 1/50.

    References

    This product has been referenced in:
    • Liu F  et al. Quantitative proteomic analysis of gastric cancer tissue reveals novel proteins in platelet-derived growth factor b signaling pathway. Oncotarget 8:22059-22075 (2017). Read more (PubMed: 28423550) »
    • Wang H  et al. Growth of MCF-7 breast cancer cells and efficacy of anti-angiogenic agents in a hydroxyethyl chitosan/glycidyl methacrylate hydrogel. Cancer Cell Int 17:55 (2017). Read more (PubMed: 28515673) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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