The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 mg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
Use at an assay dependent concentration.
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
Western blot - Anti-PDGF Receptor alpha antibody (ab96806)
All lanes : Anti-PDGF Receptor alpha antibody (ab96806) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa Observed band size: 123 kDa Additional bands at: 32 kDa, 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The PDGF Receptor Alpha protein contains a number of potential glycosylation sites (SwissProt). This might explain the migration pattern observed.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-PDGF Receptor alpha antibody (ab96806)This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, France
Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of rat brain tissue sections labeling PDGF Receptor alpha with ab96806 at 1/100 dilution. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique. Tissue was incubated with Anti-PDGF Receptor alpha antibody (ab96806) for 18 hours at 20°C in PBST. A Goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/1000 dilution.