Anti-PDGF receptor alpha + beta (phospho Y572 + Y574) antibody (ab5443)

Overview

  • Product nameAnti-PDGF receptor alpha + beta (phospho Y572 + Y574) antibody
  • Description
    Rabbit polyclonal to PDGF receptor alpha + beta (phospho Y572 + Y574)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Xenopus laevis
  • Immunogen

    Synthetic phospho peptide, containing Tyrosine 572 and 574 of Human PDGFR alpha. This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog.

  • Positive control
    • NIH3T3 cells +/- PDGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab5443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170 - 185 kDa.

Target

Anti-PDGF receptor alpha + beta (phospho Y572 + Y574) antibody images

  • Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5443 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5443 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

References for Anti-PDGF receptor alpha + beta (phospho Y572 + Y574) antibody (ab5443)

This product has been referenced in:
  • Csak T  et al. MicroRNA-155 Deficiency Attenuates Liver Steatosis and Fibrosis without Reducing Inflammation in a Mouse Model of Steatohepatitis. PLoS One 10:e0129251 (2015). WB ; Mouse . Read more (PubMed: 26042593) »
  • Paugh BS  et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res 73:6219-29 (2013). Human . Read more (PubMed: 23970477) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"