The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5-1µg for 105-8 cells. A final volume of 100 µl is recommended for staining the cell sample.
ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Staining of NIH/3T3 cell line with 0.5 µg of ab93530 followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
References for Anti-PDGF Receptor alpha (biotinylated ) antibody [APA5] (ab93530)
This product has been referenced in:
Miyawaki T et al. Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina. J Neurosci24:8124-34 (2004).
Read more (PubMed: 15371513) »
Takakura N et al. PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. J Histochem Cytochem45:883-93 (1997).
Read more (PubMed: 9199674) »