Anti-PDGF Receptor alpha (biotinylated ) antibody [APA5] (ab93530)

Overview

  • Product nameAnti-PDGF Receptor alpha (biotinylated ) antibody [APA5]
    See all PDGF Receptor alpha primary antibodies
  • Description
    Rat monoclonal [APA5] to PDGF Receptor alpha (biotinylated )
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Fusion protein containing the extracellular domain of Mouse PDGF Receptor alpha and the Fc portion of Human IgG1 purified from the culture supernatant of transfected COS1 cells.

  • Positive control
    • NIH/3T3 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab93530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 105-8 cells. A final volume of 100 µl is recommended for staining the cell sample.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha (biotinylated ) antibody [APA5] images

  • Staining of NIH/3T3 cell line with 0.5 µg of ab93530 followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

References for Anti-PDGF Receptor alpha (biotinylated ) antibody [APA5] (ab93530)

This product has been referenced in:
  • Miyawaki T  et al. Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina. J Neurosci 24:8124-34 (2004). IHC-Fr . Read more (PubMed: 15371513) »
  • Takakura N  et al. PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. J Histochem Cytochem 45:883-93 (1997). IHC-P . Read more (PubMed: 9199674) »

See all 2 Publications for this product

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