Anti-PDGF Receptor alpha (phospho Y754) antibody (ab5460)

Overview

  • Product nameAnti-PDGF Receptor alpha (phospho Y754) antibodySee all PDGF Receptor alpha primary antibodies ...
  • Description
    Rabbit polyclonal to PDGF Receptor alpha (phospho Y754)
  • SpecificityPDGFR beta (50%) has not been tested, but is not expected to react.
  • Tested applicationsICC/IF, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    Synthetic phospho peptide (Human) containing Tyrosine 754.

  • Positive control
    • NIH3T3 cells +/- PDGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab5460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent dilution. 1/50 (see Abreview)
IHC-P 1/50. (see Abreviews)
WB Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170-185 kDa.

Target

  • FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P16234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Alpha platelet derived growth factor receptor antibody
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD 140a antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF R alpha antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR A antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha (phospho Y754) antibody images

  • Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5460 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5460 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
  • ab5460 staining PDGF Receptor alpha (phospho Y754) in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H2O2, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes.

    See Abreview

  • ab5460 staining PDGF Receptor alpha (phospho Y754) in human glioblastoma multiforme brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citric acid pH 6.1. Samples were then permeabilized using 0.3% H2O2, then blocked with 0.5% BSA for 20 minutes at room temperature, followed by incubation with the primary antibody at a 1/50 dilution for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit/mouse polyclonal was used as secondary antibody. Nuclei staining with Haematoxillin (blue) and PDGF Receptor alpha staining with DAB (brown). PDGF Receptor alpha staining in the membrane of astrocytes.

    See Abreview

References for Anti-PDGF Receptor alpha (phospho Y754) antibody (ab5460)

This product has been referenced in:
  • Johnson TV  et al. Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretome. Brain N/A:N/A (2013). Read more (PubMed: 24176979) »
  • Sabha N  et al. Investigation of the In Vitro Therapeutic Efficacy of Nilotinib in Immortalized Human NF2-Null Vestibular Schwannoma Cells. PLoS One 7:e39412 (2012). WB ; Human . Read more (PubMed: 22745749) »

See all 2 Publications for this product

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I am sorry about the issues you are having with ab5460 in western blot. As we discussed I am sending you a new vial of the antibody to see if that helps resolves the situation. Your new order number i...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell line)
Specification human glioblastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X-100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Jun 17 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma cell lines)
Specification human glioblastoma cell lines
Fixative Formaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Apr 29 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (glioblastoma multiforme brain section)
Specification glioblastoma multiforme brain section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
Permeabilization Yes - 0,3 % H202
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Nov 15 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
Permeabilization Yes - 0,3 % H202
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Submitted Nov 15 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Fibroblasts)
Loading amount 150 µg
Specification Mouse Fibroblasts
Treatment PDGF 40ng/ml 5min
Gel Running Conditions Reduced Denaturing (7,5)
Blocking step PhosphoBlocker as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Oct 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"