Overview

  • Product nameAnti-PDGF Receptor beta antibody [42G12]
    See all PDGF Receptor beta primary antibodies
  • Description
    Mouse monoclonal [42G12] to PDGF Receptor beta
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Heart

Properties

Applications

Our Abpromise guarantee covers the use of ab69506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22355762
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

Anti-PDGF Receptor beta antibody [42G12] images

  • Immunohistochemistry analysis of human spleen tissue labeling PDGF Receptor beta [42G12] with ab69506 at 20µg/ml.

  • Immunohistochemistry analysis of human placenta tissue labeling PDGF Receptor beta [42G12] with ab69506 at at 20µg/ml.

  • Overlay histogram showing NIH 3T3 cells stained with ab69506 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab69506, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-PDGF Receptor beta antibody [42G12] (ab69506) at 1 µg/ml

    Lane 1 : Molecular Weight Marker
    Lane 2 : Mouse Heart Muscle lysate

    Developed using the ECL technique

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
    Note: extra bands are secondary reactivity

References for Anti-PDGF Receptor beta antibody [42G12] (ab69506)

This product has been referenced in:
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). IHC . Read more (PubMed: 25896789) »
  • Blackmore DG  et al. Growth hormone responsive neural precursor cells reside within the adult mammalian brain. Sci Rep 2:250 (2012). ICC/IF ; Mouse . Read more (PubMed: 22355762) »

See all 2 Publications for this product

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