Anti-PDGF Receptor beta antibody [APB5] (ab91066)

Overview

  • Product name
    Anti-PDGF Receptor beta antibody [APB5]
    See all PDGF Receptor beta primary antibodies
  • Description
    Rat monoclonal [APB5] to PDGF Receptor beta
  • Host species
    Rat
  • Tested applications
    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Recombinant extracellular domain of murine PDGFRB fused to Human IgG1 purified from transfected COS1 cells.

  • Positive control
    • NIH3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab91066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: Use 1 µg for 106 cells in 100 µl total staining volume.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 124 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
    • Involvement in disease
      Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
      Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
      Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
      Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta platelet derived growth factor receptor antibody
      • Beta-type platelet-derived growth factor receptor antibody
      • CD 140B antibody
      • CD140 antigen-like family member B antibody
      • CD140b antibody
      • CD140b antigen antibody
      • IBGC4 antibody
      • IMF1 antibody
      • JTK12 antibody
      • OTTHUMP00000160528 antibody
      • PDGF R beta antibody
      • PDGF-R-beta antibody
      • PDGFR 1 antibody
      • PDGFR antibody
      • PDGFR beta antibody
      • PDGFR1 antibody
      • PDGFRB antibody
      • PGFRB_HUMAN antibody
      • Platelet derived growth factor receptor 1 antibody
      • Platelet derived growth factor receptor beta antibody
      • Platelet derived growth factor receptor beta polypeptide antibody
      see all

    Images

    • Staining of NIH3T3 cells with 0.5 µg control antibody (open histogram) or 0.5 µg ab91066 (colored histogram), followed by biotin-conjugated anti-Rat IgG and Streptavidin PE.
      Total viable cells were used for analysis.

    References

    This product has been referenced in:
    • McLane LE  et al. Loss of Tuberous Sclerosis Complex1 in Adult Oligodendrocyte Progenitor Cells Enhances Axon Remyelination and Increases Myelin Thickness after a Focal Demyelination. J Neurosci 37:7534-7546 (2017). Read more (PubMed: 28694334) »
    • Anastasia A  et al. Trkb signaling in pericytes is required for cardiac microvessel stabilization. PLoS One 9:e87406 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24498100) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Prostate)
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 29 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 29 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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