Anti-PDGF Receptor beta (phospho Y1021) antibody (ab62437)

Overview

  • Product nameAnti-PDGF Receptor beta (phospho Y1021) antibodySee all PDGF Receptor beta primary antibodies ...
  • Description
    Rabbit polyclonal to PDGF Receptor beta (phospho Y1021)
  • SpecificityThis antibody detects endogenous levels of PDGF Receptor beta only when phosphorylated at tyrosine 1021.
  • Tested applicationsIHC-P, ELISA, ICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PDGF Receptor beta around the phosphorylation site of tyrosine 1021 (N-D-YP-I-I).

  • Positive control
    • Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50 - 1/100.
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P09619 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

Anti-PDGF Receptor beta (phospho Y1021) antibody images

  • ab62437 staining PDGF Receptor beta (phospho Y1021) (green) in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 1% BSA + 10% goat serum + 0.1% Triton X-100 in PBS for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - phalloidin.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab62437 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.


  • Performed under reducing conditions.

    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 seconds

    This image is courtesy of an Abreview submitted by Lars Ruether

    All lanes : PDGF Receptor beta (phospho Y1021) antibody (ab62437) at 1/100 dilution (in TBST for 12 hours at 4°C) + 100µg whole cell lysate of Human glioblastoma (A172 cells)(unstimulated Lane 1 or stimulated Lanes 2-7)

    Secondary: A Biotin-conjugated Goat polyclonal at 1/10000 dilution
    developed using the ECL technique

    Blocking Step: 5% BSA for 90 minutes at room temperature.

    See Abreview

  • ab62437 staining the PDGF Receptor beta (phospho Y1021) in epithelial cells in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 0.5% BSA for 20 minutes at room temperature; antigen retrieval was by heat mediation with citric acid (pH6.0). Samples were incubated with primary antibody (1/50 in 0.5% BSA/PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-PDGF Receptor beta (phospho Y1021) antibody (ab62437)

ab62437 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step PBS, 1% BSA, 10% goat serum, 0.1% TX-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Cell (primary embryonic epicardial)
Specification primary embryonic epicardial
Permeabilization Yes - 0.5% Triton X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 13 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue (Brian section)
Specification Brian section
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 18 2013

Thank you for your message.

I would like to reassure you that although these antibodies are recommended to be stored at -20oC, I would not expect being at 4oC for a few days to significantly affect their performance. However, I would recomme...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human non-neoplastic cortex)
Specification human non-neoplastic cortex
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrit acid pH 6.1
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (epithelia cells in mouse brain tissue)
Specification epithelia cells in mouse brain tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2010

Application Western blot
Sample Human Cell lysate - whole cell (Brain (Glioblastoma))
Loading amount 100 µg
Specification Brain (Glioblastoma)
Treatment PDGF-BB: 20 & 40 ng/ml for 5, 10, 15 min.
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Lars Rüther

Verified customer

Submitted Sep 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"