Recombinant Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021)
- Suitable for: WB, Flow Cyt (Intra), Dot blot
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-PDGFR beta (phospho Y1021) antibody [EPR2476]
See all PDGFR beta primary antibodies -
Description
Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), Dot blotmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NIH/3T3 lysates treated with Pervanadate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/50000 - 1/200000. Predicted molecular weight: 190 kDa.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Dot blot |
1/1000.
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Notes |
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WB
1/50000 - 1/200000. Predicted molecular weight: 190 kDa. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Dot blot
1/1000. |
Target
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Function
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18596 Mouse
- SwissProt: P05622 Mouse
- Unigene: 4146 Mouse
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Alternative names
- Beta platelet derived growth factor receptor antibody
- Beta-type platelet-derived growth factor receptor antibody
- CD 140B antibody
see all
Images
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All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/50000 dilution
Lane 1 : NIH/3T3 untreated
Lane 2 : NIH/3T3 treated with Pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 190 kDa -
Intracellular Intracellular Flow Cytoremtic analysis of permeabilized NIH/3T3 cells, untreated (green) or pervanadate-treated (red) using anti-PDGF Receptor beta phospho RabMab and pervanadate-treated NIH/3T3 cells using the same antibody preincubated with phospho-PDGF Receptor beta peptide (blue) or non-phospho-PDGF Receptor beta peptide (orange)
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All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/5000 dilution (Purified)
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 190 kDaBlocking/Diluting Buffer and concentration 5% NFDM/TBST
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Dot blot analysis of PDGFR beta (phospho Y1021) phospho peptide (Lane 1) and PDGFR beta non-phospho peptide (Lane 2) labeling PDGFR beta (phospho Y1021) phospho peptide with ab134048 at a dilution of 1/1000 dilution (0.54ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab134048 has not yet been referenced specifically in any publications.