Overview

  • Product nameAnti-PDHA2 antibody
    See all PDHA2 primary antibodies
  • Description
    Mouse polyclonal to PDHA2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (NP_005381.1)

Properties

Applications

Our Abpromise guarantee covers the use of ab72931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml. Protein A purification of the antibody is required before use in ICC/IF.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Tissue specificityTestis. Expressed in postmeiotic spermatogenic cells.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 1.2.4.1 antibody
      • MGC149517 antibody
      • MGC149518 antibody
      • mitochondrial antibody
      • ODPAT_HUMAN antibody
      • Pdha2 antibody
      • PDHAL antibody
      • PDHE1 A type II antibody
      • PDHE1-A type II antibody
      • Pyruvate dehydrogenase (lipoamide) alpha 2 antibody
      • Pyruvate dehydrogenase E1 component subunit alpha antibody
      • Pyruvate dehydrogenase E1 component subunit alpha, testis specific form, mitochondrial antibody
      • Pyruvate dehydrogenase, E1 alpha polypeptide, testis spec antibody
      • testis-specific form antibody
      see all

    Anti-PDHA2 antibody images

    • All lanes : Anti-PDHA2 antibody (ab72931) at 1/500 dilution

      Lane 1 : PDHA2-transfected 293T cell lysate(42.68 KDa)
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP-conjugated goat anti-mouse at 1/2500 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
    • Immunofluorescence staining of HeLa cells using protein A purified ab72931 at 10ug/ml.

    References for Anti-PDHA2 antibody (ab72931)

    ab72931 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"