Overview

  • Product name
  • Description
    Rabbit polyclonal to PDHX
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PDHX conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Corresponding to amino acid residues surrounding T11.
    Database link: O00330

  • Positive control
    • Human Colon tissue; NCI-H460 cell line lysates; 293 cell lysates, transiently transfected with the PDX1 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab178502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.

Target

  • Function
    Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
  • Involvement in disease
    Defects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349].
  • Sequence similarities
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
    • DLDBP antibody
    • E3 binding protein antibody
    • E3-binding protein antibody
    • E3BP antibody
    • Lipoyl containing pyruvate dehydrogenase complex component X antibody
    • Lipoyl-containing pyruvate dehydrogenase complex component X antibody
    • mitochondrial antibody
    • ODPX_HUMAN antibody
    • OPDX antibody
    • PDHX antibody
    • PDX 1 antibody
    • PDX1 antibody
    • Pro X antibody
    • proX antibody
    • Pyruvate dehydrogenase complex component X antibody
    • Pyruvate dehydrogenase complex lipoyl containing component X antibody
    • Pyruvate dehydrogenase complex, E3 binding protein subunit antibody
    • Pyruvate dehydrogenase protein X component antibody
    • Pyruvate dehydrogenase protein X component mitochondrial antibody
    see all

Images

  • Anti-PDHX antibody (ab178502) at 1/100 dilution + NCI H460 cell lysate at 35 µg

    Predicted band size: 54 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling PDHX with ab178502 at 10 µg/ml.

  • All lanes : Anti-PDHX antibody (ab178502) at 1/100 dilution

    Lane 1 : 293 cell lysates, non-transfected
    Lane 2 : 293 cell lysates, transiently transfected with the PDX1 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 54 kDa

References

ab178502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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