Overview

  • Product name
  • Description
    Goat polyclonal to PDHX
  • Specificity
    This antibody is expected to recognise all reported isoforms.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human PDHX aa 496-500 (C terminal).
    Sequence:

    C-KSFKANLENPIRLA


    Database link: O00330

  • Positive control
    • Human pancreas lysate. Human pancreas tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab31283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:32,000.

IHC-P Use a concentration of 1 - 3 µg/ml.

Target

  • Function
    Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
  • Involvement in disease
    Defects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349].
  • Sequence similarities
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
    • DLDBP antibody
    • E3 binding protein antibody
    • E3-binding protein antibody
    • E3BP antibody
    • Lipoyl containing pyruvate dehydrogenase complex component X antibody
    • Lipoyl-containing pyruvate dehydrogenase complex component X antibody
    • mitochondrial antibody
    • ODPX_HUMAN antibody
    • OPDX antibody
    • PDHX antibody
    • PDX 1 antibody
    • PDX1 antibody
    • Pro X antibody
    • proX antibody
    • Pyruvate dehydrogenase complex component X antibody
    • Pyruvate dehydrogenase complex lipoyl containing component X antibody
    • Pyruvate dehydrogenase complex, E3 binding protein subunit antibody
    • Pyruvate dehydrogenase protein X component antibody
    • Pyruvate dehydrogenase protein X component mitochondrial antibody
    see all

Images

  • Anti-PDHX antibody (ab31283) at 1 µg/ml (incubated for 1 hour) + Human Pancreas lysate (35µg protein in RIPA buffer)
    Developed using the ECL technique

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Sun Y  et al. Liver proteome analysis of adaptive response in rat immediately after partial hepatectomy. Proteomics 7:4398-407 (2007). Read more (PubMed: 17979177) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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