Overview

  • Product nameAnti-PDHX antibody
    See all PDHX primary antibodies
  • Description
    Goat polyclonal to PDHX
  • SpecificityThis antibody is expected to recognise all reported isoforms.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human PDHX aa 496-500 (C terminal).
    Sequence:

    C-KSFKANLENPIRLA


    Database link: O00330

  • Positive control
    • Human pancreas lysate. Human pancreas tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:32,000.

IHC-P Use a concentration of 1 - 3 µg/ml.

Target

  • FunctionRequired for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
  • Involvement in diseaseDefects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349].
  • Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
    • DLDBP antibody
    • E3 binding protein antibody
    • E3-binding protein antibody
    • E3BP antibody
    • Lipoyl containing pyruvate dehydrogenase complex component X antibody
    • Lipoyl-containing pyruvate dehydrogenase complex component X antibody
    • mitochondrial antibody
    • ODPX_HUMAN antibody
    • OPDX antibody
    • PDHX antibody
    • PDX 1 antibody
    • PDX1 antibody
    • Pro X antibody
    • proX antibody
    • Pyruvate dehydrogenase complex component X antibody
    • Pyruvate dehydrogenase complex lipoyl containing component X antibody
    • Pyruvate dehydrogenase complex, E3 binding protein subunit antibody
    • Pyruvate dehydrogenase protein X component antibody
    • Pyruvate dehydrogenase protein X component mitochondrial antibody
    see all

Anti-PDHX antibody images

  • Anti-PDHX antibody (ab31283) at 1 µg/ml (incubated for 1 hour) + Human Pancreas lysate (35µg protein in RIPA buffer)
    Developed using the ECL technique

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-PDHX antibody (ab31283)

This product has been referenced in:
  • Sun Y  et al. Liver proteome analysis of adaptive response in rat immediately after partial hepatectomy. Proteomics 7:4398-407 (2007). Read more (PubMed: 17979177) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"