Overview

  • Product nameAnti-PDHX antibody
    See all PDHX primary antibodies
  • Description
    Mouse polyclonal to PDHX
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human protein (AAH10389.1)

  • Positive control
    • WB: stomach tissue lysate or HepG2 cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionRequired for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
  • Involvement in diseaseDefects in PDHX are the cause of pyruvate dehydrogenase E3-binding protein deficiency (PDHXD) [MIM:245349].
  • Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex antibody
    • DLDBP antibody
    • E3 binding protein antibody
    • E3-binding protein antibody
    • E3BP antibody
    • Lipoyl containing pyruvate dehydrogenase complex component X antibody
    • Lipoyl-containing pyruvate dehydrogenase complex component X antibody
    • mitochondrial antibody
    • ODPX_HUMAN antibody
    • OPDX antibody
    • PDHX antibody
    • PDX 1 antibody
    • PDX1 antibody
    • Pro X antibody
    • proX antibody
    • Pyruvate dehydrogenase complex component X antibody
    • Pyruvate dehydrogenase complex lipoyl containing component X antibody
    • Pyruvate dehydrogenase complex, E3 binding protein subunit antibody
    • Pyruvate dehydrogenase protein X component antibody
    • Pyruvate dehydrogenase protein X component mitochondrial antibody
    see all

Anti-PDHX antibody images

  • Anti-PDHX antibody (ab88601) at 1 µg/ml + Human stomach lysate at 50 µg

    Predicted band size : 54 kDa
  • Anti-PDHX antibody (ab88601) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 54 kDa
  • All lanes : Anti-PDHX antibody (ab88601) at 1 µg/ml

    Lane 1 : lysate from PDHX transfected 293T cells
    Lane 2 : lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab88601 at 10 µg/ml detecting PDHX in paraformaldehyde fixed HeLa cells by immunofluorescence.

References for Anti-PDHX antibody (ab88601)

ab88601 has not yet been referenced specifically in any publications.

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