Anti-PDI antibody [1D3] (Phycoerythrin) (ab115650)

Overview

  • Product nameAnti-PDI antibody [1D3] (Phycoerythrin)
    See all PDI primary antibodies
  • Description
    Mouse monoclonal [1D3] to PDI (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Synthetic peptide corresponding to the sequence near the C terminal of Rat PDI.

  • Positive control
    • Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab115650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificityHighly expressed in pancreas (at protein level).
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • P4HB antibody
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

Anti-PDI antibody [1D3] (Phycoerythrin) images

  • ab115650 at 10µg/ml staining PDI in 106 Jurkat cells by Flow cytometry (shown in red).
    Grey: unstained;Yellow: isotype control.

References for Anti-PDI antibody [1D3] (Phycoerythrin) (ab115650)

ab115650 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I have contacted the lab for a detailed protocol.
Am I assuming correctly that you are doing flow cytometry?
As for the Jurkat cells used as positive control, we know that 10^6 (1 million) cells were used and th...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"