Anti-PDI antibody [1D3] (Phycoerythrin) (ab115650)

Overview

  • Product name
    Anti-PDI antibody [1D3] (Phycoerythrin)
    See all PDI primary antibodies
  • Description
    Mouse monoclonal [1D3] to PDI (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Synthetic peptide corresponding to the sequence near the C terminal of Rat PDI.

  • Positive control
    • Jurkat cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab115650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificity
    Highly expressed in pancreas (at protein level).
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

Images

  • ab115650 at 10µg/ml staining PDI in 106 Jurkat cells by Flow cytometry (shown in red).
    Grey: unstained;Yellow: isotype control.

References

ab115650 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your reply.
I have contacted the lab for a detailed protocol.
Am I assuming correctly that you are doing flow cytometry?
As for the Jurkat cells used as positive control, we know that 10^6 (1 million) cells were used and th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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