Overview

  • Product name
  • Description
    Goat polyclonal to PDI
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Monkey, Gorilla, Common marmoset, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to Human PDI aa 129-141 (internal sequence) (Cysteine residue).
    Sequence:

    THPEEYTGPRDAE


    Database link: Q13087

  • Positive control
    • Human pancreas tissue; Human pancreas lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab189368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 58 kDa).

The observed molecular weight corresponds to the glycosylated form.

IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificity
    Highly expressed in pancreas (at protein level).
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localization
    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • Pancreas-specific protein disulfide isomerase antibody
    • pancreatic protein disulfide isomerase antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • protein disulfide isomerase family A member 2 antibody
    • protein disulfide isomerase, pancreatic antibody
    • protein disulfide isomerase-associated 2 antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

Images

  • Anti-PDI antibody (ab189368) at 0.03 µg/ml + Human Pancreas lysate at 35 µg

    Predicted band size: 58 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling PDI with ab189368 at 3.75 µg/ml.

References

ab189368 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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