Overview

  • Product nameAnti-PDI antibodySee all PDI primary antibodies ...
  • Description
    Rabbit polyclonal to PDI
  • SpecificityPDI runs at 61.2 kD on samples from human placenta. In samples from rat liver, it runs at 59 kD, but 3 other bands may appear due to protein degradation - these run at 58.9 kD, 55.2 kD and 53.8 kD.
  • Tested applicationsICC, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, African Green Monkey, Chinese Hamster
  • Immunogen

    Full length protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab3672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
WB
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC: 1/50 - 1/100.
    ICC/IF: 1/600 (PMID 19059915).
    IHC-Fr: 1/100.
    WB: 1/1000. Detects a band of approximately ~60 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
    • Tissue specificityHighly expressed in pancreas (at protein level).
    • Sequence similaritiesBelongs to the protein disulfide isomerase family.
      Contains 2 thioredoxin domains.
    • Post-translational
      modifications
      The disulfide-linked homodimer exhibits an enhanced chaperone activity.
      Glycosylated.
    • Cellular localizationEndoplasmic reticulum lumen.
    • Information by UniProt
    • Database links
    • Alternative names
      • P4HB antibody
      • Pancreas specific protein disulfide isomerase antibody
      • Pancreas-specific protein disulfide isomerase antibody
      • Pancreatic protein disulfide isomerase antibody
      • PDA2 antibody
      • PDI antibody
      • PDIA2 antibody
      • PDIA2_HUMAN antibody
      • PDIP antibody
      • PDIR antibody
      • Protein disulfide isomerase A2 antibody
      • Protein disulfide isomerase antibody
      • Protein disulfide isomerase associated 2 antibody
      • Protein disulfide isomerase family A member 2 antibody
      • Protein disulfide isomerase pancreatic antibody
      • Protein disulfide-isomerase A2 antibody
      • Rho GDP dissociation inhibitor gamma antibody
      see all

    Anti-PDI antibody images

    • Anti-PDI antibody (ab3672) at 1/1000 dilution + PC12 whole cell lysate at 20 µg

      Secondary
      HRP conjugated swine anti-rabbit antibody
      developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 36 kDa,48 kDa,58 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 2 minutes

      See Abreview

    • ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr.  Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C.  A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary.

      See Abreview

    • ab3672 staining PDI - ER in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary.

      See Abreview

    • ICC/IF image of ab3672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3672, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

    References for Anti-PDI antibody (ab3672)

    This product has been referenced in:
    • Zins SR  et al. The human alpha defensin HD5 neutralizes JC polyomavirus infection by reducing endoplasmic reticulum traffic and stabilizing the viral capsid. J Virol 88:948-60 (2014). Human . Read more (PubMed: 24198413) »
    • Kemter E  et al. Type of uromodulin mutation and allelic status influence onset and severity of uromodulin-associated kidney disease in mice. Hum Mol Genet 22:4148-63 (2013). Mouse . Read more (PubMed: 23748428) »

    See all 9 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK293)
    Specification HEK293
    Fixative Formaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Mr. Armen Petrosyan

    Verified customer

    Submitted Dec 03 2012

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Chinese Hamster Cell (ovary)
    Specification ovary
    Fixative Methanol
    Permeabilization Yes - 0.1% Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 08 2010

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HaCaT keratinocyte)
    Specification HaCaT keratinocyte
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.25% triton X-100
    Blocking step 2.5% BSA plus 1% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2009

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Cortical oligodendrocytes in vibratome sections)
    Specification Cortical oligodendrocytes in vibratome sections
    Fixative Somogy's. PFA + picric acid PLP also, PFA + lysine + periodate
    Permeabilization Yes - TX100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Username

    Dr. Guillermo Estivill-Torrus

    Verified customer

    Submitted Oct 31 2007

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa S3)
    Total protein in input 500 µg
    Specification HeLa S3
    Immuno-precipitation step Protein A
    Username

    Abcam user community

    Verified customer

    Submitted Oct 30 2007

    Application Western blot
    Sample Hamster Cell lysate - whole cell (CHO cell line)
    Loading amount 30 µg
    Specification CHO cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 20 2007

    Application Western blot
    Sample Mouse Cell lysate - whole cell (3T3 cell line)
    Loading amount 30 µg
    Specification 3T3 cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 20 2007

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
    Loading amount 30 µg
    Specification Cos-7 cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 20 2007

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (adrenal tumour (phaeochromocytoma))
    Specification adrenal tumour (phaeochromocytoma)
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 10 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"