Overview

  • Product nameAnti-PDI antibody - ER Marker
    See all PDI primary antibodies
  • Description
    Rabbit polyclonal to PDI - ER Marker
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Synthetic peptide corresponding to Human PDI aa 400-500 conjugated to keyhole limpet haemocyanin.
    Database link: P07237
    (Peptide available as ab31810)

  • Positive control
    • This antibody gave a positive signal in the lysates: Human Brain Whole Tissue Lysate, Human Kidney Whole Tissue Lysate, Human Liver Whole Tissue Lysate and Human Testes Whole Tissue Lysate, Mouse Liver Whole Tissue Lysate; Rat Liver Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PDI peptide (ab31810).
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionActs as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.
  • Tissue specificityHighly expressed in pancreas (at protein level).
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Post-translational
    modifications
    The disulfide-linked homodimer exhibits an enhanced chaperone activity.
    Glycosylated.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • P4HB antibody
    • Pancreas specific protein disulfide isomerase antibody
    • Pancreas-specific protein disulfide isomerase antibody
    • Pancreatic protein disulfide isomerase antibody
    • PDA2 antibody
    • PDI antibody
    • PDIA2 antibody
    • PDIA2_HUMAN antibody
    • PDIp antibody
    • PDIR antibody
    • Protein disulfide isomerase A2 antibody
    • Protein disulfide isomerase antibody
    • Protein disulfide isomerase associated 2 antibody
    • Protein disulfide isomerase family A member 2 antibody
    • Protein disulfide isomerase pancreatic antibody
    • Protein disulfide-isomerase A2 antibody
    • Rho GDP dissociation inhibitor gamma antibody
    see all

Anti-PDI antibody - ER Marker images

  • PDI was immunoprecipitated using 0.5mg Mouse Pancreas tissue lysate, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Pancreas tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31811.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 57kDa, non specific - as present in control (lane 2); 55kDa: We are confident this was due to slight lane contamination and the band seen in the IP lane is our target of interest; PDI

  • All lanes : Anti-PDI antibody - ER Marker (ab31811) at 1 µg/ml

    Lane 1 : Human Brain Whole Tissue Lysate
    Lane 2 : Human Kidney Whole Tissue Lysate at 20 µg
    Lane 3 : Human Liver Whole Tissue Lysate at 20 µg
    Lane 4 : Human Testes Whole Tissue Lysate at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Several smaller bands are seen in Human Liver Whole Tissue Lysate. These are predicted to be break-down products of PDI; the banding pattern is consistent with that observed in Liver lysates for other available PDI antibodies.
  • Image courtesy of Human Protein Atlas

    ab31811 staining PDI in Human gall bladder. The paraffin embedded tissue was incubated with ab31811 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31811 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab31811 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31811, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and MCF7 cells.

  • All lanes : Anti-PDI antibody - ER Marker (ab31811) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 30 seconds
  • IHC image of ab31811 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31811, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PDI antibody - ER Marker (ab31811)

This product has been referenced in:
  • Langer F  et al. Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase. Blood 121:2324-35 (2013). Human . Read more (PubMed: 23315166) »
  • Gaertner A  et al. In vitro functional analyses of arrhythmogenic right ventricular cardiomyopathy-associated desmoglein-2-missense variations. PLoS One 7:e47097 (2012). WB ; Human . Read more (PubMed: 23071725) »

See all 2 Publications for this product

Product Wall

Sorry for the dely in getting back to you. I now have further information concerning Anti-PDI antibody (ab31811). The human brain tissue lysate used for the Western blot presented on the datasheet of ab31811 was taken from the parietal lobe. Th...

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Thank you for contacting us again. When I said that ab31811 had not been charaterised as well as ab2792 I simply meant that this antibody had not been used and referenced in as many publications and had not had as many reviews by our other customer...

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Thank you for contacting us. I have looked into the products that we have available for the species and application you are interested in (Western blotting with human brain samples) for PDI and Connexin. 1. PDI There are two antibodies which lo...

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