Overview

  • Product nameAnti-PDIA6 antibody
    See all PDIA6 primary antibodies
  • Description
    Rabbit polyclonal to PDIA6
  • Tested applicationsSuitable for: ICC/IF, IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat, Hamster, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human PDIA6 aa 476-492.
    Sequence:

    DIDLSDVELDDLGKDEL


    (Peptide available as ab11432)

  • Positive control
    • Human fibrosarcoma HT 1080 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab11432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).

Target

  • FunctionMay function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
  • Tissue specificityExpressed in platelets (at protein level).
  • Sequence similaritiesBelongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localizationEndoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum protein 5 antibody
    • ER protein 5 antibody
    • ERp5 antibody
    • P5 antibody
    • Pdia6 antibody
    • PDIA6_HUMAN antibody
    • Protein disulfide isomerase A6 antibody
    • Protein disulfide isomerase associated 6 antibody
    • Protein disulfide isomerase family A member 6 antibody
    • Protein disulfide isomerase P5 antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A6 antibody
    • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
    • Thioredoxin domain containing protein 7 antibody
    • Thioredoxin domain-containing protein 7 antibody
    • TXNDC7 antibody
    see all

Anti-PDIA6 antibody images

  • PDIA6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PDIA6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab11432.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 48kDa; PDIA6

  • Western blot of PDIA6 in HT 1080 cell lysate using ab11432. 

  • ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PDIA6 antibody (ab11432)

This product has been referenced in:
  • Groenendyk J  et al. Interplay between the oxidoreductase PDIA6 and microRNA-322 controls the response to disrupted endoplasmic reticulum calcium homeostasis. Sci Signal 7:ra54 (2014). WB . Read more (PubMed: 24917591) »
  • Wang X  et al. An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic target to inhibit shedding. Biochem Biophys Res Commun 387:476-81 (2009). WB ; Human . Read more (PubMed: 19615970) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"