Overview

  • Product name
  • Description
    Rabbit polyclonal to PDIA6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide within residues: KNRPEDYQGG RTGEAIVDAA LSALRQLVKD RLGGRSGGYS SGKQGRSDSS , corresponding to N terminal amino acids 108-157 of Human PDIA6 (NP_005733)

  • Positive control
    • 721_B cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83456 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 2 µg/ml.
IP Use a concentration of 2 µg/ml.

Target

  • Function
    May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
  • Tissue specificity
    Expressed in platelets (at protein level).
  • Sequence similarities
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endoplasmic reticulum protein 5 antibody
    • ER protein 5 antibody
    • ERp5 antibody
    • P5 antibody
    • Pdia6 antibody
    • PDIA6_HUMAN antibody
    • Protein disulfide isomerase A6 antibody
    • Protein disulfide isomerase associated 6 antibody
    • Protein disulfide isomerase family A member 6 antibody
    • Protein disulfide isomerase P5 antibody
    • Protein disulfide isomerase related protein antibody
    • Protein disulfide-isomerase A6 antibody
    • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
    • Thioredoxin domain containing protein 7 antibody
    • Thioredoxin domain-containing protein 7 antibody
    • TXNDC7 antibody
    see all

Images

  • Anti-PDIA6 antibody (ab83456) at 1 µg/ml + 721_B cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunofluorescence analysis of Human brain stem cells staining PDIA6 with ab83456 at 2 µg/ml.

  • IP using ab83456 at 2 ug/ml of Human NT2 cell lysate

References

ab83456 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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