Overview

  • Product nameAnti-PDK2 antibody
    See all PDK2 primary antibodies
  • Description
    Mouse monoclonal to PDK2
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: HIGSIDPNCN VSEVVKDAYD MAKLLCDKYY MASPDLEIQE INAANSKQPI HMVYVPSHLY HMLFELFKNA MRATVESHES SLILPPIKVM , corresponding to amino acids 187-276 of Human PDK2

Properties

Applications

Our Abpromise guarantee covers the use of ab55575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityExpressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
    • mitochondrial antibody
    • PDHK2 antibody
    • PDK2 antibody
    • PDK2_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoform 2 antibody
    • Pyruvate dehydrogenase kinase, isozyme 2 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
    see all

Anti-PDK2 antibody images

  • PDK2 antibody (ab55575) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab55575 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55575, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-PDK2 antibody (ab55575)

ab55575 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (PDK2)
Specification PDK2
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type Direct
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Submitted Mar 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"