Overview

  • Product name
  • Description
    Rabbit polyclonal to PDK3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 7-290 of Human PDK3 (Uniprot ID: Q15120).

  • Positive control
    • HepG2 whole cell lysate; HepG2 cells; Human Ca922 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Found almost exclusively in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
    • CMTX6 antibody
    • GS1-358P8.4 antibody
    • mitochondrial antibody
    • PDK3 antibody
    • PDK3_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoenzyme 3 antibody
    • Pyruvate dehydrogenase kinase isoform 3 antibody
    • Pyruvate dehydrogenase kinase isozyme 3 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody
    see all

Images

  • Anti-PDK3 antibody (ab154549) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue labeling PDK3 with ab154549 at 1/100 dilution.
  • Immunofluorescent analysis of methanol-fixed HepG2 cells labeling PDK3 with ab154549 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Wang LY  et al. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Rep 16:3016-27 (2016). Read more (PubMed: 27626669) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Hamster Cell lysate - whole cell (Chinese Hamster Ovary Cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
300000 cells
Specification
Chinese Hamster Ovary Cells
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

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Verified customer

Submitted Feb 14 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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