Overview

  • Product name
    Anti-PDK3 antibody - C-terminal
    See all PDK3 primary antibodies
  • Description
    Rabbit polyclonal to PDK3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse PDK3 aa 386-414 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q922H2

  • Positive control
    • Mouse liver tissue lysate; Mouse heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab172874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.

Target

  • Function
    Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificity
    Found almost exclusively in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
    • CMTX6 antibody
    • GS1-358P8.4 antibody
    • mitochondrial antibody
    • PDK3 antibody
    • PDK3_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoenzyme 3 antibody
    • Pyruvate dehydrogenase kinase isoform 3 antibody
    • Pyruvate dehydrogenase kinase isozyme 3 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Mouse heart tissue labeling PDK3 using ab172874 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-PDK3 antibody - C-terminal (ab172874) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size: 47 kDa

References

ab172874 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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