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Rabbit monoclonal [EP12309] to PDK3
Predicted to work with:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PDK3 aa 250-350. The exact sequence is proprietary.
Database link: Q15120
Mouse heart and kidney tissue. Rat Heart tissue and C6 lysates. Raw264.7 cells and cell lysates.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Found almost exclusively in heart and skeletal muscle.
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Information by UniProt
[Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
Flow Cytometry - Anti-PDK3 [EP12309] antibody (ab182574)
Flow cytometric analysis of Raw26.7 cells (paraformaldehyde-fixed, 2%) labeling PDK3 with ab182574 at 1/400 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.
Western blot - Anti-PDK3 [EP12309] antibody (ab182574)
All lanes : Anti-PDK3 antibody [EP12309] (ab182574) at 1/2000 dilution Lane 1 : Mouse heart lysate Lane 2 : Mouse kidney lysate Lane 3 : Rat heart lysate Lane 4 : C6 lysate Lane 5 : Raw264.7 lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution Predicted band size: 47 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"