Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab88063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityUbiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all

Anti-PDK4 antibody images

  • Anti-PDK4 antibody (ab88063) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-PDK4 antibody (ab88063)

ab88063 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"