Overview

  • Product nameAnti-PDK4 antibody
    See all PDK4 primary antibodies
  • Description
    Rabbit polyclonal to PDK4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the C terminal region of Human PDK4

  • Positive control
    • WB: Human skeletal muscle lysate. IHC: human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab89295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/250.

Target

  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityUbiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
    • FLJ40832 antibody
    • mitochondrial antibody
    • Pdk4 antibody
    • PDK4_HUMAN antibody
    • Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial antibody
    • Pyruvate dehydrogenase kinase 4 antibody
    • Pyruvate dehydrogenase kinase isoenzyme 4 antibody
    • Pyruvate dehydrogenase kinase isoform 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 antibody
    • Pyruvate dehydrogenase kinase isozyme 4 mitochondrial antibody
    see all

Anti-PDK4 antibody images

  • Anti-PDK4 antibody (ab89295) at 1/500 dilution + Human fetal skeletal muscle lysate

    Predicted band size : 46 kDa
  • Anti-PDK4 antibody (ab89295) at 1/500 dilution + Human A549 (lung carcinoma) cell line.

    Predicted band size : 46 kDa
  • Ab89295 staining PDK4 antibody in human gastrointestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • Left panel: ab89295 at 1/100 dilution showing cytoplasmic staining of PDK4 in formalin-fixed, paraffin-embedded human fetal skeletal tissue by Immunohistochemistry.
    Right panel: same, after blocking with the immunizing peptide.

References for Anti-PDK4 antibody (ab89295)

This product has been referenced in:
  • Zhang Y  et al. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS One 11:e0150454 (2016). Mouse . Read more (PubMed: 26930489) »
  • Frier BC  et al. Interactions between the consumption of a high-fat diet and fasting in the regulation of fatty acid oxidation enzyme gene expression: an evaluation of potential mechanisms. Am J Physiol Regul Integr Comp Physiol 300:R212-21 (2011). WB ; Rat . Read more (PubMed: 21084676) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab89295.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"