Overview

  • Product nameAnti-PDLIM1 antibody
    See all PDLIM1 primary antibodies
  • Description
    Rabbit polyclonal to PDLIM1
  • Specificityab64971 detects endogenous levels of total PDLIM1 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal region of human PDLIM1.

  • Positive control
    • Human heart tissue, HepG2 cells and extracts from Jurkat, COLO205, 293, HepG2 and HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100. Do not perform antigen retrieval.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP Use at an assay dependent concentration.

Target

  • FunctionCytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
  • Tissue specificityStrongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • C terminal LIM domain protein 1 antibody
    • C-terminal LIM domain protein 1 antibody
    • Carboxy terminal LIM domain protein 1 antibody
    • CLIM 1 antibody
    • CLIM1 antibody
    • CLP 36 antibody
    • CLP36 antibody
    • Elfin antibody
    • Enigma antibody
    • Enigma homolog antibody
    • hCLIM 1 antibody
    • hCLIM1 antibody
    • LIM domain protein CLP 36 antibody
    • LIM domain protein CLP-36 antibody
    • LIM domain protein CLP36 antibody
    • PDLI1_HUMAN antibody
    • PDLIM 1 antibody
    • Pdlim1 antibody
    • PDZ and LIM domain 1 antibody
    • PDZ and LIM domain protein 1 antibody
    see all

Anti-PDLIM1 antibody images

  • PDLIM1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to PDLIM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64971.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 36kDa: PDLIM1.
  • All lanes : Anti-PDLIM1 antibody (ab64971) at 1/500 dilution

    Lane 1 : Jurkat cell extract; without immunizing peptide
    Lane 2 : COLO205 cell extract; without immunizing peptide
    Lane 3 : 293 cell extract; without immunizing peptide
    Lane 4 : HepG2 cell extract; without immunizing peptide
    Lane 5 : HUVEC cell extract; without immunizing peptide
    Lane 6 : HUVEC cell extract; with immunizing peptide


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 22 kDa,28 kDa,30 kDa,32 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of PDLIM1 in in paraffin embedded human heart tissue using ab64971 at 1/50 dilution; in the presence (left) and absence (right) of the immunizing peptide.
  • Immunofluorescence analysis of PDLIM1 in HepG2 cells, with ab64971 (1/500 diltion). in the presence (left) and absence (right) of the immunizing peptide.
  • Immunofluorescence analysis of Human trabecular meshwork cells, staining PDLIM1 with ab64971.

    Left panel: PDLIM1 staining alone. Right panel: PDLIM1 staining (red) merged with F-actin staining (green).

    Cells were treated with dexamethasone, before fixing in paraformaldehyde and permeabilizing wih 0.2% Triton X-100. Cells were incubated with primary antibody (2 µg/ml) and AlexaFluor®488-conjugated phalloidin. PDLIM1 staining was detected using an AlexaFluor®546-conjugated goat anti-rabbit IgG.

References for Anti-PDLIM1 antibody (ab64971)

This product has been referenced in:
  • Clark R  et al. Comparative Genomic and Proteomic Analysis of Cytoskeletal Changes inDexamethasone-Treated Trabecular Meshwork Cells. Mol Cell Proteomics : (2012). ICC/IF . Read more (PubMed: 23105009) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. Unfortunately, the immunogen is not commercially available. Please let us know if you have any other questions on concerns.

Thank you for contacting us. While the immunogen sequence for ab64971 is considered proprietary, we can confirm that it does not overlap with the immunogen for ab115842.

The immunogen for ab115842 shows 78% homology with mouse PDLIM1 and may ...

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Thank you for contacting Abcam.

The amino acid range of this immunogen is aa******** of human PDLIM1 with accession number O00151 (http://www.uniprot.org/uniprot/O00151).

Please let me know if there is anything else I can help you w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"