Overview

  • Product name
    Anti-PDLIM1 antibody [EPR7187]
    See all PDLIM1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7187] to PDLIM1
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human PDLIM1 (O00151).

  • Positive control
    • Jurkat cell line lysate HeLa cell line lysate and cells 293T cell line lysate Caco-2 cell line lysate Saos-2 cell line lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
    • Tissue specificity
      Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
    • Sequence similarities
      Contains 1 LIM zinc-binding domain.
      Contains 1 PDZ (DHR) domain.
    • Cellular localization
      Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • C terminal LIM domain protein 1 antibody
      • C-terminal LIM domain protein 1 antibody
      • Carboxy terminal LIM domain protein 1 antibody
      • CLIM 1 antibody
      • CLIM1 antibody
      • CLP 36 antibody
      • CLP36 antibody
      • Elfin antibody
      • Enigma antibody
      • Enigma homolog antibody
      • hCLIM 1 antibody
      • hCLIM1 antibody
      • LIM domain protein CLP 36 antibody
      • LIM domain protein CLP-36 antibody
      • LIM domain protein CLP36 antibody
      • PDLI1_HUMAN antibody
      • PDLIM 1 antibody
      • Pdlim1 antibody
      • PDZ and LIM domain 1 antibody
      • PDZ and LIM domain protein 1 antibody
      see all

    Images

    • All lanes : Anti-PDLIM1 antibody [EPR7187] (ab126628) at 1/1000 dilution

      Lane 1 : Jurkat cell line lysate
      Lane 2 : HeLa cell line lysate
      Lane 3 : 293T cell line lysate
      Lane 4 : Caco 2 cell line lysate
      Lane 5 : Saos 2 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size : 36 kDa
    • ab126628, at 1/250, staining PDLIM1 in HeLa cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . Read more (PubMed: 27078885) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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