Overview

  • Product nameAnti-PDLIM2 antibody [10C4]
    See all PDLIM2 primary antibodies
  • Description
    Mouse monoclonal [10C4] to PDLIM2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human PDLIM2 produced in HEK293T cells (NP_067643).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY PDLIM2 cDNA; HepG2, A549, COS7, Jurkat, MDCK and MCF7 cell extracts; Human tonsil tissue; COS7 cells transiently transfected by pCMV6-ENTRY PDLIM2.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 37 kDa.
IHC-P 1/150.
ICC/IF 1/100.

Target

  • FunctionProbable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.
  • Sequence similaritiesContains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localizationCytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ34715 antibody
    • MYSTIQUE antibody
    • OTTHUMP00000162522 antibody
    • OTTHUMP00000205197 antibody
    • OTTHUMP00000205198 antibody
    • OTTHUMP00000205199 antibody
    • OTTHUMP00000205200 antibody
    • OTTHUMP00000205202 antibody
    • OTTHUMP00000205203 antibody
    • PDLI2_HUMAN antibody
    • Pdlim2 antibody
    • PDZ and LIM domain 2 (mystique) antibody
    • PDZ and LIM domain protein 2 antibody
    • PDZ-LIM protein mystique antibody
    • SLIM antibody
    • Stat-interacting LIM protein antibody
    see all

Anti-PDLIM2 antibody [10C4] images

  • All lanes : Anti-PDLIM2 antibody [10C4] (ab119243) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PDLIM2 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 37 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203156)
  • All lanes : Anti-PDLIM2 antibody [10C4] (ab119243) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell line extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 37 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203147)
  • ab119243, at 1/150, staining PDLIM2 in paraffin embedded Human tonsil by Immunohistochemistry.
  • ab119243, at 1/100, staining PDLIM2 in COS7 cells transiently transfected with pCMV6-ENTRY PDLIM2 by Immunofluorescence.

References for Anti-PDLIM2 antibody [10C4] (ab119243)

ab119243 has not yet been referenced specifically in any publications.

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