Overview

  • Product name
  • Description
    Mouse polyclonal to PDLIM2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length PDLIM2 protein (Human)

  • Positive control
    • PDLIM2 transfected 293T cell lysate and human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36, 38 kDa (predicted molecular weight: 37 kDa).

Target

  • Function
    Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ34715 antibody
    • MYSTIQUE antibody
    • OTTHUMP00000162522 antibody
    • OTTHUMP00000205197 antibody
    • OTTHUMP00000205198 antibody
    • OTTHUMP00000205199 antibody
    • OTTHUMP00000205200 antibody
    • OTTHUMP00000205202 antibody
    • OTTHUMP00000205203 antibody
    • PDLI2_HUMAN antibody
    • Pdlim2 antibody
    • PDZ and LIM domain 2 (mystique) antibody
    • PDZ and LIM domain protein 2 antibody
    • PDZ-LIM protein mystique antibody
    • SLIM antibody
    • Stat-interacting LIM protein antibody
    see all

Images

  • Anti-PDLIM2 antibody (ab68220) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PDLIM2 antibody (ab68220) at 1/500 dilution

    Lane 1 : PDLIM2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Wurster KD  et al. Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma. Leukemia 31:602-613 (2017). WB ; Human . Read more (PubMed: 27538486) »
  • Vanoirbeek E  et al. PDLIM2 expression is driven by vitamin D and is involved in the pro-adhesion, and anti-migration and -invasion activity of vitamin D. Oncogene N/A:N/A (2013). Human . Read more (PubMed: 23584482) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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