Overview

  • Product nameAnti-PDLIM7 antibody
    See all PDLIM7 primary antibodies
  • Description
    Rabbit polyclonal to PDLIM7
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)

  • Positive control
    • Hela cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
  • Tissue specificityIsoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.
  • Sequence similaritiesContains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • DomainThe LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
    The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
    Anchored to cell periphery via its N-terminal PDZ domain.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110003B01Rik antibody
    • Enigma antibody
    • LIM domain protein antibody
    • LIM domain protein enigma antibody
    • LIM mineralization protein 1 antibody
    • Lim mineralization protein 3 antibody
    • LIM mineralization protein antibody
    • LMP 1 antibody
    • LMP antibody
    • LMP1 antibody
    • LMP3 antibody
    • PDLI7_HUMAN antibody
    • PDLIM 7 antibody
    • Pdlim7 antibody
    • PDZ and LIM doamin protein 7 antibody
    • PDZ and LIM domain 7 antibody
    • PDZ and LIM domain protein 7 antibody
    • Protein enigma antibody
    see all

Anti-PDLIM7 antibody images

  • All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa


    Exposure time : 30 seconds
  • Detection of PDLIM7 by Western blot of Immunprecipitate.
    ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 seconds.

References for Anti-PDLIM7 antibody (ab86065)

ab86065 has not yet been referenced specifically in any publications.

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