The immunogen sequence for ab182153 shares 86% homology with the mouse PDLIM7 protein however in our hands this antibody did not work in mouse and rat samples in western blot (no bands detected). In some human cell lines, in addition to the band of interest detected at 50 kDa, this product also bound to a protein at ~37-40 kDa in weight. For best results it may be preferable to use a harsh lysis protocol for preparation of cell or tissue lysates. Please see notes in the western blot image below.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.
The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4. The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5. Anchored to cell periphery via its N-terminal PDZ domain.
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
Immunohistochemical analysis of paraffin-embedded Human infiltrating ductal breast carcinoma tissue labeling PDLIM7 with ab182153 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PDLIM7 with ab182153 at 1/60 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.