Recombinant
RabMAb

Anti-PDLIM7 antibody [EPR13171] (ab182153)

Overview

  • Product name
    Anti-PDLIM7 antibody [EPR13171]
    See all PDLIM7 primary antibodies
  • Description
    Rabbit monoclonal [EPR13171] to PDLIM7
  • Specificity
    The immunogen sequence for ab182153 shares 86% homology with the mouse PDLIM7 protein however in our hands this antibody did not work in mouse and rat samples in western blot (no bands detected). In some human cell lines, in addition to the band of interest detected at 50 kDa, this product also bound to a protein at ~37-40 kDa in weight. For best results it may be preferable to use a harsh lysis protocol for preparation of cell or tissue lysates. Please see notes in the western blot image below.
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human PDLIM7 aa 100-250. The exact sequence is proprietary.
    Database link: Q9NR12

  • Positive control
    • HeLa and Jurkat cell lysates; Human infiltrating ductal breast carcinoma tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab182153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
  • Tissue specificity
    Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver.
  • Sequence similarities
    Contains 3 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
    The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
    Anchored to cell periphery via its N-terminal PDZ domain.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110003B01Rik antibody
    • Enigma antibody
    • LIM domain protein antibody
    • LIM domain protein enigma antibody
    • LIM mineralization protein 1 antibody
    • Lim mineralization protein 3 antibody
    • LIM mineralization protein antibody
    • LMP 1 antibody
    • LMP antibody
    • LMP1 antibody
    • LMP3 antibody
    • PDLI7_HUMAN antibody
    • PDLIM 7 antibody
    • Pdlim7 antibody
    • PDZ and LIM doamin protein 7 antibody
    • PDZ and LIM domain 7 antibody
    • PDZ and LIM domain protein 7 antibody
    • Protein enigma antibody
    see all

Images

  • Lane 1 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/10000 dilution
    Lanes 2 - 4 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/2000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 : WI-38 (human lung) whole cell lysate
    Lane 4 : SK-OV-3 (human adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit IgG (H+L) peroxidase conjugated. at 1/1000 dilution

    Predicted band size : 50 kDa


    Exposure time : 3 minutes

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer:5% NFDM/TBST

    Lane 1 exposure time: 1 minute

    Lanes 2 - 4 exposure time: 3 minutes

  • Immunohistochemical analysis of paraffin-embedded Human infiltrating ductal breast carcinoma tissue labeling PDLIM7 with ab182153 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PDLIM7 with ab182153 at 1/60 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab182153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Aortic smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
25 µg
Specification
Aortic smooth muscle cells
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Omer Shkedi

Verified customer

Submitted Jun 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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