Overview

  • Product name
  • Description
    Rabbit polyclonal to PDPK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human PDPK1 aa 277-556 (C terminal).
    Database link: O15530

  • Positive control
    • LOVO and mouse lung extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab186870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificity
    Appears to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localization
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling PDPK1 with ab186870 at 1/200 dilution. Heat-mediated antigen retrieval with Tris-EDTA pH 9.0 for 15 minutes, 95-100oC.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186870. Blue DAPI for nuclear staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling PDPK1 with ab186870 at 1/200 dilution. Heat-mediated antigen retrieval with Tris-EDTA pH 9.0 for 15 minutes, 95-100oC.

  • All lanes : Anti-PDPK1 antibody (ab186870) at 1/500 dilution

    Lane 1 : LOVO extract
    Lane 2 : Mouse lung extract

    Predicted band size: 63 kDa

References

ab186870 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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