Anti-PDPK1 (phospho S241) antibody (ab131098)

Overview

  • Product name
    Anti-PDPK1 (phospho S241) antibody
    See all PDPK1 primary antibodies
  • Description
    Rabbit polyclonal to PDPK1 (phospho S241)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to a region around phosphorylation site Serine 241 (

    A-N-S(p)-F-V

    ) of Human PDPK1 (NP_002604.1; UniProt O15530).

  • Positive control
    • Extracts from 293 and PC12 cell treated with EGF; HeLa cells, Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificity
    Appears to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localization
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Images

  • All lanes : Anti-PDPK1 (phospho S241) antibody (ab131098) at 1/500 dilution

    Lane 1 : 293 cell extract, untreated
    Lane 2 : 293 cell extract treated with EGF
    Lane 3 : PC12 cell extract, untreated
    Lane 4 : PC12 cell extract treated with EGF

    Predicted band size: 63 kDa

  • Immunohistochemical analysis of PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue, using ab131098 at a dilution of 1/50. Image on the right was preincubated with blocking peptide.
  • Immunofluorescence analysis of PDPK1 (phospho S241) in methanol fixed HeLa cells, using ab131098 at a dilution of 1/100.

References

ab131098 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab131098.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up