RabMAb

Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460)

Overview

  • Product nameAnti-PDPK1 (phospho S241) antibody [EPR336(2)]See all PDPK1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR336(2)] to PDPK1 (phospho S241)
  • Specificityab109460 only detects PDPK1 phosphorylated at Serine 241.
  • Tested applicationsWB, IP, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 241 of Human PDPK1.

  • Positive control
    • 293T cell lysate; Human breast carcinoma tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • Tissue specificityAppears to be expressed ubiquitously.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Target information above from: UniProt accession O15530 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Anti-PDPK1 (phospho S241) antibody [EPR336(2)] images

    • All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : 293T cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 63 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab109460, at a 1/100 dilution, staining PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    References for Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460)

    This product has been referenced in:

    See 1 Publication for this product

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